Thursday, October 31, 2013

Second attempt

The extraction for the the tomato and the carrot came out really well but there was a slight problem. When I was extracting the DNA from the tomato, I didn't yield a high amount of DNA so I had roughly 0.1mL of tomato DNA and after running it through the Gel I was left with practically nothing. So the bad news is that I will have to extract more DNA from the tomato. The second part of the bad news is that when I was pippetting I accidentally tore the well in the gel where I was placing the DNA and didn't notice it so nothing came up on the gel except for the carrots DNA. Next step is to extract more DNA from more plants and then run then through the PCR to amplify it.

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